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Title Full Name Email Phone Seminar Department Motivation Supervisor Thesis Research Programme Description Research Programme Keywords
Mr Sinomncedi Magida 0834813883 statics Biotechnology, University of Johannesburg
Biotechnology, University of Johannesburg
Biotechnology, University of Johannesburg
Biotechnology, University of Johannesburg
I am passionate about bioinformatics and all the various tools that are used within the field. I have opted to apply for this course as it would be an ideal skill for me to acquire to be a better bioinformatics specialist. I have done a bit of Python and have recently completed a course on Linux with ACGT and am looking forward to completing this course as well. Dr Mahloro Hope Serepa-Dlamini Evaluation of multi-metal tolerance and plant-promotion characteristics of bacterial endophytes isolated from Solanum nigrum Endophytes are defined as any micro-organisms, bacteria, fungi etc., which form relationships with plants. In this study, we hypothesise that since this plant can be able to bioremediate metals, its bacterial endophytes can bioremediate metals as well. The plant has been chosen as previous studies have shown its ability to host endophytic bacteria with heavy metal bioremediation potential.




Linux Programming
R
Mr Sinomncedi Magida 0834813883 statics Biotechnology, University of Johannesburg
Biotechnology, University of Johannesburg
Biotechnology, University of Johannesburg
Biotechnology, University of Johannesburg
I am passionate and curious about bioinformatics tools and the many ways they can be applied when studying biological data. I am interested in acquiring this skill to add to my own repertoire as I have already completed an introductory course to Linux with ACGT recently. This course offers a great opportunity for me to acquire an invaluable skill. Dr Mahloro Hope Serepa-Dlamini Evaluation of multi-metal tolerance and plant-promotion characteristics of bacterial endophytes isolated from Solanum nigrum Endophytes are defined as any micro-organisms, bacteria, fungi etc., which form relationships with plants. n this study, we hypothesise that since this plant can be able to bioremediate metals, its bacterial endophytes can bioremediate metals as well. Solanum nigrum is known to bioremediate some heavy metals. The plant has been chosen as previous studies have shown its ability to host endophytic bacteria with heavy metal bioremediation potential.




Linux Programming
R
Miss Tayla Rabie 0769021075 statics Biochemistry, University of Pretoria
Bioinformatics is now being used for many applications including analysis of gene expression, prediction of protein structure and function, as well as metabolic pathways. Having a greater understanding of these applications and their uses will help me to study various genes or proteins and their interactions in the plasmodium life cycle as well as in other living systems. Using these tools it may also allow for the discovery of new drug targets or the molecular interaction of different protein structures. This course will give me the knowledge to increase my understanding of the different biological processes and pathways within a cell. Prof Lyn-Marie Birkholtz Genetic manipulation of histone deacetylases SAP18 and SIR2A in Plasmodium falciparum Malaria parasites are increasingly developing resistance to current drug treatments, emphasizing the continued need for new drugs and biological processes targeting the disease-causing parasite, Plasmodium falciparum. This includes the unique and unusual epigenome of the parasite, which uses epigenetic mechanisms to regulate both asexual proliferation and sexual differentiation. This study investigates the putative histone deacetylase I complex protein, SAP18, and a member of the histone deacetylase III family, SIR2A through genetic manipulation strategies. These proteins are thought to regulate histone acetylation, thereby allowing activation or repression of genes in a very specific manner. Selection linked integration
Plasmodium falciparum
Dr David Livingstone Nsibo 0840401521 statics Plant Science , University of Pretoria
In my study I will use population genetics analysis tools. The measures of genotypic diversity based on two indices: Shannon-Weiner Index of MLG diversity (I); and Simpson’s complement index of MLG diversity will be computed using the R package poppr. Using the clone-corrected dataset, Nei’s unbiased gene diversity will also be computed using R packages. Other analyses under this software will include mantel test, principal component analyses and population structure analyses. Based on the importance of this R package for my study, I am sure this training will equip me with these skills for its successful execution. Prof Nigel Barker The population genetics and physiological race diversity of Exserohilum turcicum, a foliar fungal pathogen of maize in smallholder and emerging commercial farms in the Eastern Cape. Northern leaf blight (NLB) is ranked as the number one foliar fungal disease of maize with potential of causing more than ten percent global yield losses especially in cases where it is inaccurately diagnosed and managed ineffectively. Exserohilum turcicum is the causal pathogen of NLB causing up to 90% yield losses especially under severe infections during silking and grain fill. Management of NLB is through the cultural practices, breeding for qualitative or quantitative resistance and fungicide application. Most of these strategies remain unsuccessful due to the limited knowledge in the genetic diversity of E. turcicum populations. Therefore, effective management of this pathogen, must be guided by the knowledge of populations of this pathogen and how they have evolved in response to their host, and the changing environments. Very few population genetics studies of E. turcicum have been conducted in South Africa all of which reveal that E. turcicum is a genetically diverse pathogen. Little remains known about the genetic diversity of the pathogen in other countries north of South Africa. In this project, we will thus determine the genetic diversity of E. turcicum and the evolutionary factors driving its diversity among smallholder and emerging commercial farms in Kenya. poppr
Adegenet
Dr. Nyeleti Manganyi-Ntabankulu 0727419876 statics ACGT, University of Pretoria
I would appreciate if I am considered for this opportunity. I know and strongly believe that this course will assist me as a post-graduate student and a scientist to improve my data management skills. This tool will assist me both in school and in the workplace. As a master’s student, I am expected to do analysis of my data, without knowledge and tools of which data analysis software to use it can be challenging to begin and become a productive as one would want. Kindly consider me for the course and I will really appreciate and use it opportunity to the best of my capability. Dr. Moerane Udder Health Status in cattle belonging to small-scale farmers in Eastern Cape and Gauteng provinces. The aim of this study was to determine the prevalence of udder damage focusing on injury and parasites; assess the general udder conformation of cattle by focusing on conformation and depth, the levels of Somatic Cell Count amongst lactating cows, prevalence and antimicrobial resistance of mastitogenic bacteria isolated from lactating cows, and udder management measures implemented by small-scale farmers in order to get a general proportion of udder health in cattle belonging to small-scale farmers in Eastern Cape and Gauteng provinces. It is of great importance that small-scale farmers transform and be commercial in order to assist with food security in these provinces and South Africa as a whole. However, farmers in these areas struggle to meet the market demands that commercial sector expects from them in order to be up in the bar.






Dr Mahloro Hope Serepa-Dlamini statics Biotechnology, University of Johannesburg
The course is an ideal opportunity for me as a Bacterial Genomics and biotechnology researcher. We currently have analysed metagenomic data of some of the bacterial endophyte communities and we would like to do statistics on the analysed data using R and other related stats softwares. Due to continual and growing research done in science, I am accustomed to working with new information and actively improve on my knowledge and skills, and of those around me. The information, knowledge and skills from this course will also be of benefit to my colleagues and students at the University of Johannesburg. None Senior Lecturer I head the Bacterial Genomics and Bacteriology Research Group. My research group focuses on Bacterial Genomics and Biotechnology with various projects centred on studying the biology, physiology, evolution and bioprospects of symbiotic bacterial species isolated from entomopathogenic nematodes and medicinal plant species obtained in the country. We equally take a deeper look at the colonization, diversity, biocontrol properties, genomics and metagenomics of bacterial species with further insights to development of biopesticides and drugs from these bacterial species to limit environmental pollution and antimicrobial resistance, respectively.







Miss Mariska Naude 0832623949 statics Biochemistry, University of Pretoria
With this letter I (Mariska Naude) would like to state my motivation to participate in the online course “edX Statistics and R - data analysis in the life sciences”. I am currently a PhD Biochemistry student at the University of Pretoria (South Africa) and my study focus on understanding Plasmodium falciparum parasite biology to facilitate development of screening assays for quicker and easier screening of compounds with transmission-blocking activity as this will contribute to malaria elimination strategies. This course is especially relevant to my professional area as statistics is always part of data analysis. Although I have experience with statistics, I would like to obtain more confidence in the use of statistics, and expand on my statistical knowledge, specifically for life sciences. Furthermore, with the growing opportunities of R for data analysis and statistical functionality, I would also like to become more knowledgeable on using R. Therefore, I would highly appreciate to be given an opportunity to attend this course. Lyn-Marie Birkholtz Profiling gametocytocidal compounds for malaria elimination strategies Plasmodium falciparum parasites are the causative agent of the most severe form of malaria and concerns regarding the emergence of parasite resistance to current antimalarials exist. There is a need for the development of novel antimalarials that specifically target the transmissible, gametocyte stages of the parasite that will ensure complete clearance of these stages and ultimately blocking transmission, which is currently not achieved with compounds that target both asexual and gametocyte stages. Screening cascades for the identification of such a compound is time-consuming and labour intensive due to the dependence of the development of mosquito stages. My study aims to develop screening assays for quicker and easier screening of compounds with transmission-blocking activity as this will contribute to drug discovery initiatives.


Mr Louis Ramoba 0152684059 statics Biochemistry, Microbiology and Biotechnology , University of Limpopo
Biochemistry, Microbiology and Biotechnology , University of Limpopo
In my project study, i am expected to compare my analysis statistically. I lack statistical analysis knowledge and guidance to help me see through my research results analysis. I will be grateful if my application can be considered. Prof KLM Mokganedi Optimisation of marula wine starter culture fermentation and maturation: A strategy to evaluate the effect on chemical, nutrition and sensory properties. The project it is about optimising the process of producing marula wine with commercial qualities, evaluating the chemical, nutritional and sensory attributes. Different starter cultures isolated and studied from previous spontaneous fermentation of marula wine will be used to ferment the marula juice at two different controlled temperature. Post fermentation the fermented juice will be allowed to mature at two different temperature. Continuous sampling will be applied in one day interval. The sampling will be carried out in duplicate. All the samples will be evaluated for chemical, nutritional and sensory qualities.

















Mr Louis Ramoba 0152684059 statics Biochemistry, Microbiology and Biotechnology , University of Limpopo
Biochemistry, Microbiology and Biotechnology , University of Limpopo
In my project study, i am expected to compare my analysis statistically. I lack statistical analysis knowledge and guidance to help me see through my research results analysis. I will be grateful if my application can be considered. Prof KLM Mokganedi Optimisation of marula wine starter culture fermentation and maturation: A strategy to evaluate the effect on chemical, nutrition and sensory properties. The project it is about optimising the process of producing marula wine with commercial qualities, evaluating the chemical, nutritional and sensory attributes. Different starter cultures isolated and studied from previous spontaneous fermentation of marula wine will be used to ferment the marula juice at two different controlled temperature. Post fermentation the fermented juice will be allowed to mature at two different temperature. Continuous sampling will be applied in one day interval. The sampling will be carried out in duplicate. All the samples will be evaluated for chemical, nutritional and sensory qualities.

















MS Dipoelo Mogashoa 0767048986 statics Biochemistry, Microbiology and Biotechnology , University of Limpopo
Biochemistry, Microbiology and Biotechnology , University of Limpopo
I come across as a mature young lady, who is insightful and confident. I am interactive and engaging at most times. I am committed to my research work for masters’ studies and enjoying the field I am researching. I am dedicated to my studies; meticulous and has excellent communication and interpersonal skills. Therefore, attending this course will help increase my knowledge on most of the microbiology concepts as well as my researching skills. It will help improve the knowledge I have on data analysis. Prof KLM Moganedi Investigation of the microbiological, nutritional and health properties of mageu produced from different flours. There is a need to utilise the available crops such as maize, millet and sorghum due to the prevalent food insecurity, large populations and diseases in Africa. It is important to develop and improve the nutritive value of these food products in order to sustain and nourish the health of the African population. About 80% of the South African population rely on maize as staple food and the increase in population, poor soil fertility, water resource and limited land are presently distressing maize production capacity. Mageu is produced from maize and leftover maize porridge in African communities. The studies of Katongole (2008) and Simatende (2015) described mageu as a fermented non-alcoholic maize-based drink that is used as weaning food for babies and consumed at different ceremonies. The proposed study explores an introduction of different flours,(e.g sorghum) as an alternative cereal meal to maize in the making of traditional African food products.












MS Dipoelo Mogashoa 0767048986 statics Biochemistry, Microbiology and Biotechnology , University of Limpopo
Biochemistry, Microbiology and Biotechnology , University of Limpopo
I come across as a mature young lady, who is insightful and confident. I am interactive and engaging at most times. I am committed to my research work for masters’ studies and enjoying the field I am researching. I am dedicated to my studies; meticulous and has excellent communication and interpersonal skills. Therefore, attending this course will help increase my knowledge on most of the microbiology concepts as well as my researching skills. It will help improve the knowledge I have on data analysis. Prof KLM Moganedi Investigation of the microbiological, nutritional and health properties of mageu produced from different flours. With the prevalent food insecurity, large populations and diseases in Africa, there is a need to utilize the available crops such as maize, millet and sorghum to develop and improve the nutritious food to sustain and nourish the health of the population. For about 80% of the South African population rely on maize as the staple food product and the increase in population, poor soil fertility, water resource and limited land are presently distressing maize production capacity (Mutsamba, 2020; Zhao, 2020). The study of (Katongole, 2008; Simatende, 2015), describes mageu as one of the fermented non-alcoholic maize-based drink that is used as weaning product for babies and consumed at different ceremonies. Therefore, the development of maize- based fermented beverage with a mixture of different flours will follow the traditional method of production, thereby preserving culture.












Mr Sinomncedi Magida 0834813883 statics Biotechnology, University of Johannesburg
Biotechnology, University of Johannesburg
Biotechnology, University of Johannesburg
Biotechnology, University of Johannesburg
I am very passionate about bioinformatics and the different tools available for this subject. As a field that produces a lot of data, i believe statistical tools are indispensable for the study of Life Science subjects and this is why I would love to do this course. Dr Mahloro Hope Serepa-Dlamini Evaluation of multi-metal tolerance and plant-promotion characteristics of bacterial endophytes isolated from Solanum nigrum Aim: The aim of the present study is to assess the bioremediation potential of endophytic bacteria isolated from Solanum nigrum. Objectives: 1. To isolate, identify and characterize bacterial endophytes from Solanum nigrum. 2. To evaluate the plant promoting characteristics of the endophytes 3. To evaluate the multi-metal tolerance of the endophytes 4. To optimize growth parameters such for improved bioremediation of acid mine drainage.




Linux Programming
R
Mr Sizwe Tshabalala 0843608795 statics Biochemistry, University of Pretoria
Scientific data is practically meaningless without statistical validation. Statistical analyses is the only way we can determine if the differences were are seeing are due to some system we are studying and are not just differences due to biological variation. This makes a course like this quite important. I am personally studying the levels of expression between two line and need to perform statistical analyses to determine if there is a real difference in expression levels. Prof Lyn-Marie Birkholtz Functional evaluation of the Myb1 transcription factor during gametocytogenesis in Plasmodium falciparum The main aim of the project is to understand the role of Myb1 in the gametocytes of Plasmodium falciparum. There is some evidence that this protein is expressed in the early stages of gametocytogenesis and functional analysis will be do determine the role this protein may have in gametocyte development and sexual dimorphism
Ms Ramatsobane Mapheto 0718274086 statics Biochemistry, Microbiology and Biotechnology , University of Limpopo
I believe I am a suitable candidate for this course as my educational background position me as an academic but with limited statistical knowledge. I have completed a BSc (Life Sciences) degree as well as BSc Honours degree in Microbiology both from the University of Limpopo. I am currently registered for MSc in Microbiology. I have basic knowledge of scientific concepts and my honours degree has made me familiar with a numerous scientific skills. My goal is to become a well established researcher and I believe this opportunity will enable be to fulfil this goal. Prof Kgabo Moganedi Optimisation f marula wine from Mankweng The project involves the production of high alcohol wine that is inoculated with starter cultures. It consists of Primary fermentation,Secondary fermentation as well as ageing where clarification and stabilisation will take place. Nutriotional, chemical , alcohol analysis as well as sensory characteristics will be evaluated.




Miss Asavela Kama 0614053227 statics School of Molecular and Cell Biology, University of the Witwatersrand
In South Africa, Bioinformatics is a subject that is only offered at third year level. This implies that my biological science theory is good however, I had limited computational experience for my honors and masters research. Furthermore, this disadvantage is discouraging to most students hence there are only a few bioinformaticians. My proudest moments in life include obtaining an Honors degree in Applied Bioinformatics, under lockdown restrictions due to the COVID19 pandemic. This means that I had less resources as I was working from home, relying mostly on Youtube tutorials, an old PC and occasional availability of electricity. I should be selected for the course because my passion for computational biology fuels my willingness to learn. I am one of the few black female scientists and I aim to produce information that elevates the standard of research in my third-world country. I aim to be an inspiration to the masses of high school students from townships and rural areas who have not yet received the privilege of even using a computer. The skills that I will obtain from the course will improve the quality of my work as well as my confidence in presenting it to the world. Dr NIkki Gentle Differential splicing analysis of PMA treated pro-monocytic cell lines Monocytes and macrophages are cells that provide defense against pathogen infections through phagocytosis and releasing inflammatory signals. Subtypes of these cells are selectively activated and recruited for their tasks. Viruses such as HIV and SARS-CoV2 can selectively use these cells for virulence and persistence in the host. Therefore, specificity in characterizing monocytes and macrophages is important for understanding viral interaction and effective drug design. However, monocytes and macrophages are classified according to their cell surface receptor signatures and this fails to capture all the heterogeneous subtypes of these cells. In contrast, this study analyzes these cells according to cell specific alternative splicing events using pro-monocytic cells. Such cells exhibit monocytic characteristics and are treated with phorbol myristate acetate (PMA) to induce a macrophage phenotype. The aim is to identify and characterize alternative splicing events in three PMA treated pro-monocytic cell lines, HL60, U937 and THP1, in order to reveal the heterogeneity that is within monocytes and macrophages. IsoformSwitchAnalyzeR will be used to identify the differential isoform usage between monocytes and macrophages. SUPPA2 will be used to identify the differential inclusion of alternative splicing events between monocytes and macrophages. Lastly, the computationally identified events will be verified in vitro, using RT-PCR. Animal Biotechnnology-Novel virus particles as delivery systems for recombinant animal vaccines
Health Biotechnology-Tuberculosis
Health Biotechnology- studies on HIV epitopes as candidates for drug and vaccine development
Mr Kenneth Malatji 0710750728 statics Onderstepoort Veterinary Institute, ARC
Onderstepoort Veterinary Institute, ARC
Onderstepoort Veterinary Institute, ARC
I am doing my PhD in Animal production studies, it is important for me to study this course because R is very important in data science because of its versatility in the field of statistics. It is used in the field of data science when the task requires special analysis of data for standalone or distributed computing. In my study there will be Focus group discussions that will employ participatory mapping of animal movements and consist of 10-20 livestock farmers from each dip tank. A descriptive statistical analysis will be used to describe the basics of animal movement data for the period of the study. Network analysis will be performed using both directed and un-directed binary networks. Softwares will be used to construct the social network analysis of cattle and small ruminant movement and presented on an ArcGIS 10.4 (ESRI, USA) map using geographical coordinates of reported outbreak locations. A conditional logistic regression analysis stratified by matched set with adjustment for covariates will be used in SPSS version 24.0 (SPSS Inc., Chicago, IL, USA) to estimate the effects of animal movements on reported FMD outbreaks for 2005-2020. Questions will also be posed concerning the reasons for animal movements. Prof G.Fosgate Epidemiological approaches for the improved control of foot-and-mouth disease (FMD) in the FMD Protection Zone of Limpopo Province, in South Africa Foot-and-mouth disease (FMD) is an economically important disease of livestock and a global threat to national and international trade. The epidemiology of FMD in southern Africa is different from the rest of the world because of the important role wildlife species play in disease maintenance and transmission. The African buffalo is a reservoir for the Southern African Territories (SAT) serotypes 1-3 of FMDV. South Africa should take the global lead in the epidemiology and control of FMD at the human/domestic/wildlife interface. A lack of human capital is the only thing that is preventing South Africa from becoming the global leader in FMD epidemiology and control at the wildlife interface. The protection of wildlife resources in South Africa should be a priority from a purely economic perspective in addition to the intangible benefits to society through shared cultural values and national pride. The large economic costs attributed to FMD outbreaks and the loss of the OIE recognized free zone highlights the potential benefits of developing innovative control methods for FMD. The significance of the proposed research is that the results will provide evidence for the creation of effective control methods for the prevention of FMD outbreaks within South Africa.








Mr Kenneth Malatji 0710750728 statics Onderstepoort Veterinary Institute, ARC
Onderstepoort Veterinary Institute, ARC
Onderstepoort Veterinary Institute, ARC
I am doing my PhD in Animal production studies, it is important for me to study this course because R is very important in data science because of its versatility in the field of statistics. It is used in the field of data science when the task requires special analysis of data for standalone or distributed computing. In my study there will be Focus group discussions that will employ participatory mapping of animal movements and consist of 10-20 livestock farmers from each dip tank. A descriptive statistical analysis will be used to describe the basics of animal movement data for the period of the study. Network analysis will be performed using both directed and un-directed binary networks. Softwares will be used to construct the social network analysis of cattle and small ruminant movement and presented on an ArcGIS 10.4 (ESRI, USA) map using geographical coordinates of reported outbreak locations. A conditional logistic regression analysis stratified by matched set with adjustment for covariates will be used in SPSS version 24.0 (SPSS Inc., Chicago, IL, USA) to estimate the effects of animal movements on reported FMD outbreaks for 2005-2020. Questions will also be posed concerning the reasons for animal movements. Prof G.Fosgate Epidemiological approaches for the improved control of foot-and-mouth disease (FMD) in the FMD Protection Zone of Limpopo Province, in South Africa Foot-and-mouth disease (FMD) is an economically important disease of livestock and a global threat to national and international trade. The epidemiology of FMD in southern Africa is different from the rest of the world because of the important role wildlife species play in disease maintenance and transmission. The African buffalo is a reservoir for the Southern African Territories (SAT) serotypes 1-3 of FMDV. South Africa should take the global lead in the epidemiology and control of FMD at the human/domestic/wildlife interface. A lack of human capital is the only thing that is preventing South Africa from becoming the global leader in FMD epidemiology and control at the wildlife interface. The protection of wildlife resources in South Africa should be a priority from a purely economic perspective in addition to the intangible benefits to society through shared cultural values and national pride. The large economic costs attributed to FMD outbreaks and the loss of the OIE recognized free zone highlights the potential benefits of developing innovative control methods for FMD. The significance of the proposed research is that the results will provide evidence for the creation of effective control methods for the prevention of FMD outbreaks within South Africa.








Mr Kenneth Malatji 0710750728 statics Onderstepoort Veterinary Institute, ARC
Onderstepoort Veterinary Institute, ARC
Onderstepoort Veterinary Institute, ARC
I am doing my PhD in Animal production studies, it is important for me to study this course because R is very important in data science because of its versatility in the field of statistics. It is used in the field of data science when the task requires special analysis of data for standalone or distributed computing. In my study there will be Focus group discussions that will employ participatory mapping of animal movements and consist of 10-20 livestock farmers from each dip tank. A descriptive statistical analysis will be used to describe the basics of animal movement data for the period of the study. Network analysis will be performed using both directed and un-directed binary networks. Softwares will be used to construct the social network analysis of cattle and small ruminant movement and presented on an ArcGIS 10.4 (ESRI, USA) map using geographical coordinates of reported outbreak locations. A conditional logistic regression analysis stratified by matched set with adjustment for covariates will be used in SPSS version 24.0 (SPSS Inc., Chicago, IL, USA) to estimate the effects of animal movements on reported FMD outbreaks for 2005-2020. Questions will also be posed concerning the reasons for animal movements. Prof G.Fosgate Epidemiological approaches for the improved control of foot-and-mouth disease (FMD) in the FMD Protection Zone of Limpopo Province, in South Africa Foot-and-mouth disease (FMD) is an economically important disease of livestock and a global threat to national and international trade. The epidemiology of FMD in southern Africa is different from the rest of the world because of the important role wildlife species play in disease maintenance and transmission. The African buffalo is a reservoir for the Southern African Territories (SAT) serotypes 1-3 of FMDV. South Africa should take the global lead in the epidemiology and control of FMD at the human/domestic/wildlife interface. A lack of human capital is the only thing that is preventing South Africa from becoming the global leader in FMD epidemiology and control at the wildlife interface. The protection of wildlife resources in South Africa should be a priority from a purely economic perspective in addition to the intangible benefits to society through shared cultural values and national pride. The large economic costs attributed to FMD outbreaks and the loss of the OIE recognized free zone highlights the potential benefits of developing innovative control methods for FMD. The significance of the proposed research is that the results will provide evidence for the creation of effective control methods for the prevention of FMD outbreaks within South Africa.








Ms Sebengwane Lischen Kekana 0792773035 statics Biochemistry, Microbiology and Biotechnology , University of Limpopo
I am Ms. Sebengwane Lischen Kekana, currently studying at the University of Limpopo. I am a Masters student of microbiology under indigenous knowledge systems. My research requires calculations, sampling and comparisons from the total volume of the marula wines at different stages. Comparisons of present and previous data obtained from my study and literature respectively are very important. My study focuses mainly in the differences of the temperature optimized to produce wine samples. The method depend on three stages, growing of the starter cultures at different volumes, fermentation of marula juice and maturation of the wine. Hence all requires comparisons by statistical analysis. Prof.KLM Moganedi Optimization of temperature on the fermented test and traditional wine Aim: To compare the test( low sugar) wine to the spontaneous wine. Objectives: Comparative analysis between the low alcohol fermented marula wine under optimized temperatures to the traditional marula wine Research method Nutritional components: carbohydrates, proteins, minerals, vitamins and total fats. Protective properties: pH, total acids, volatile acids and alcohol content. Microbial properties: comparison of different microorganisms obtained from the wine after maturation. Sensory evaluations: Analyses using a 5-point hedoic-system for investigation of organoleptic properties such as taste, aroma and appearance. Statistical analysis: One way ANOVA will be used to check the significant difference between the two wines. Conclusion: The analyses are to prove the availability of indigenous fruits to produce different wines which are mostly consumed in the world.









Ms Kanganwiro Mugwanda +277911419386 statics ACGT, CSIR
I am in the first year of my PhD in Microbiology. I would benefit immensely from learning about statistical analysis, particularly the use of R in these early stages of my study. I believe this course would allow me to design my study optimally. Dr Deepak Govindaraj Synthetic circuits and metabolic engineering of Lactobacillus casei to deliver a COVID-19 vaccine The main objective of the project is to engineer a minimal and synthetic version of the probiotic bacterium Lactobacillus casei as a chassis for human metabolic therapy. A whole-cell model of L. casei based on organism-specific theoretical data will be validated experimentally and used to predict the impact of genome modifications. Synthetic biology tools will be used to engineer a therapeutic chassis that is reliable and safe for human use. The chassis will be evaluated for robust growth and ability to surface display or secrete therapeutic proteins. The study may lead to a deeper understanding of the genetic dynamics of L. casei and, if successful, pave the way for modelling of more complex bacteria and rational genetic engineering.




Miss Veronica Phetla +27723765392 statics Being in the field of research and epidemiology, statistics analysis form part of the bases for a well rounded outcome to many of the studies we do especially on occurrence rate of infectious disease in wildlife across different regions in order to make predictions and prevent spread. Dr Mamohale Application of DNA barcoding for identification and differentiation of gastrointestinal (GIT) parasites (Phylum Nematoda) of wildlife The aim of the study is to identify and characterize gastrointestinal parasites in selected wildlife species using DNA barcoding. To achieve the aim of the study, the following objectives will be achieved: • Molecular identification of the following GIT nematodes (Toxocara cati, Toxascaris leonina, Trichostrongylus sp., Nematodirus spathiger, Ostertagia ostertagii, Trichuris sp., and Haemonchus contortus) • Characterization of infections by Sanger sequencing of selected PCR positive samples • Phylogenetic analysis to determine evolutionary relationships between sequences of the GIT nematodes of wildlife at the NZG.







Dr Elizabeth Chimera 0972486368 statics Veterinary and Tropical Diseases , University of Pretoria
I am a young driven researcher who is determined to advance her statistical and technical skills and knowledge on use of different software. I am also a PhD student who will be carrying out different kinds of analysis during my studies and i believe that taking part in this course will equip me with the relevant knowledge and skill that i will need to carry out my analysis accurately and effectively. This traning course will also help me obtain valid results that will be scientifically sound and will used to contribute to the livestock sector in Malawi and in every institution that i may find myself in. It will be such a privilege to be awarded the opportunity to take part in this course. Geoffrey Fosgate Spatio-temporal patterns, risk factors and economic impact of foot-and-mouth disease in Malawi between 1957 and 2019 A modeling study based on retrospective data was conducted to establish the spatio-temporal distribution, detect clusters and determine the risk factors associated with FMD in Malawi. The SaTscan software (http://www.satscan.org/) based on a Bernoulli probability model as described by Kulldorff will be used for the cluster detection. A knowledge-driven spatial model based on a multi-criteria decision analysis approach (MCDA) using Idrisi 17.0 Selva GIS and Image Processing Software (Clark Labs, Worcester, USA) will be used to identify risk FMD risk areas. A stochastic model will be used for estimation of economical FMD impacts. A spreadsheet will be constructed in Microsoft Excel and calculations will be made using Monte-Carlo simulations and a cost-benefit analysis (CBA) will be performed for a set of proposed alternative FMD control strategies using the current control situation strategies and assess the economic implications of three FMD control scenarios. The findings will provide insight into the pattern of FMD occurrence in Malawi that will promote informed decisions for the progressive control of FMD in the country. Knowledge of disease impact will help when deciding on the level of expenditure that can be justified by a disease control programme.







Ms Elise Bonnet 0829533073 statics Medical Virology, University of Pretoria
Internationally, arboviruses are emerging with new and evolving strains. This emphasises the need to research the potential burden of disease, both locally and possibly internationally, with infections in animals and humans. With thorough future research these viruses could also be explored for possible veterinary benefits such as vaccine development and viral therapeutics. By attending this course, not only will I experience a whole congregation of experts in various scientific pursuits, but also have an amazing and tremendous opportunity to learn from them in the field of genome science and bioinformatics; truly a dream come true. I am determined to make the most of said opportunity If I were chosen. The skills I could learn will be unparalleled as it will help me towards my desired goal of pursuing a future in Academia and Research to further add to the ocean of information that we share. Marietjie Venter The molecular and serological characterization of Middelburg virus and other alphaviruses in South Africa using a One Health approach. My project is titled ‘The Molecular and Serological Characterization of Middelburg virus and other lesser-known alphaviruses using a One Health approach’. In summary, for one of my main project objectives, a surveillance network of private and state veterinarians, virologists and veterinary pathologists collaborate to investigate cases of arboviral disease in animals across South Africa. Samples are collected from domesticated animals such as felines, canines, horses, and livestock. These along with wildlife samples are submitted to the Zoonotic Arbo- and Respiratory Virus research unit (ZARV) in the form of biofluids and tissues. This includes serum, plasma, ethylene diamine-tetra acetic acid (EDTA), cerebrospinal fluid, central nervous system samples (CNS) and clotted blood. RNA was extracted under biosafety laboratory level 3 (BSL3) conditions and amplified using a RT-PCR with published degenerate primers and probes specific to Middelburg virus (MIDV) and Sindbis virus (SINV). At the end of my project I will be able to conduct phylogenetic analysis of the individual genes as well as the complete genomes of MIDV and any other alphaviruses identified through RT-PCR to investigate the molecular and clinical epidemiology of the identified strains, as well as identify any recombination events.
Mrs Danelle Venter 0793428990 statics Veterinary and Tropical Diseases , University of Pretoria
As statistics forms an integral part of reporting research findings, I would like to learn which tests are appropriate for specific study designs. For my Honors and MSc, I have used a statistician to analyze my data, but for my PhD, I would like to do it myself and be able to teach my students (which I supervise), how to do it themselves as well. Furthermore, the world is moving into the technical age and learning R is a very important skill for scientists and will enable me to effectively combine and analyze my research data, whilst reporting it appropriately. Statistics and coding is something I have not been practicing in my professional career and I feel that it would be very beneficial for me to learn these skills. Prof Anita Michel PhD: Transmission of tuberculosis between livestock and humans; Departmental project: Environmental pollutants – Health effects and Food safety It is clear that tuberculosis (TB) can be transferred between species, although pathogenicity and virulence of the transmission and adaptation of these strains are still being investigated. Due to the high prevalence of human TB (hTB) in KZN, it is integral to not only investigate bovine TB (bTB) transmission from cattle to humans, but also hTB transmission from humans to cattle. Although the transmission of bTB to humans are still largely unknown, the transmission of human TB (hTB) to cattle are even less explored. Evidence shows instances where cattle have been infected with hTB via the respiratory and genitourinary tracts. In 2014, Mittal et al. described the possibility of “reverse zoonosis”, where M. tuberculosis was observed in lung tissue of cattle. This is particularly concerning as it could indicate possible adaptation of M. tuberculosis strains in cattle (Mittal et al., 2014). It is of critical importance to the fight against tuberculosis that the prevalence of M. tuberculosis in cattle in a high-risk area such as KZN is determined. Furthermore, the strains have to be identified to determine pathogenicity and virulence. This data will be essential in the battle against multi-drug resistant strain development and the prevention of infection at the livestock/human interface. Bacterial culture
The intradermal tuberculin test
Interferon gamma assay
ELISA-antibodies and lymphocyte transformation
Molecular diagnostic and genetic typing techniques