Whole Transcriptome Sequencing Data Analysis workshop 01 – 08th February 2019 University of Pretoria

RNA Seq aims to unravel the sum of all transcripts in an organism at any given moment in time and can give important clues to changes occurring in an organism following a variety of environmental cues or life stage transitions. The ACGT, in conjunction with the Universities of Zurich, Pretoria and the Agricultural Research Council, will host a whole transcriptome sequencing (RNA sequencing or RNA Seq) data analysis workshop for researchers in Pretoria during the first week of February 2019. In preparation for the workshop, there will also be introductory sessions to Linux and R for those accepted to attend the workshop.

Important dates:

·      01 February 2019 (Friday) – Introduction to Linux
·      04 February 2019 (Monday) – Introduction to R
·      05-08 February 2019 (Tuesday to Friday) – RNA Seq WorkshopTopics:

·      Theoretical aspects (technologies and applications, experimental design, batch effects, dimension reduction, clustering, QC, limma, normalization)
·      Environment setup (Organizing files, running QC on datasets)
·      Quantification
·      Differential expression
·      Advanced topics (Geneset testing, single cell pre-processing, workflow)
·      One-on-one sessions with delegates to address their specific queries or to clarify any issues that may have arisen.

The Facilitators:

The workshop will include a mix of lectures and hands-on practical sessions facilitated by renowned and expert academics. The facilitators will include:
·      Professor Mark Robinson: Professor of Statistical Genomics at the Institute of Molecular Life Sciences at the University of Zurich in Switzerland – Research interests are in the application of statistical methods and data science to experimental data with biological applications within the context of genomics data types.
·      Doctor Simone Tiberi: Post-Doctoral Research Fellow, also from the University of Zurich, who works on on the development of cutting-edge statistical methods in bioinformatics, mostly for bulk and single-cell RNA-seq data.
·      Professor Fourie Joubert: Bioinformaticist and Director of the Centre for Bioinformatics and Computational Biology at the University of Pretoria.
·      Doctor Rian Pierneef: Bioinformatics Research Scientist at the Biotechnology Platform of the Agricultural Research Council.Application Process:

Should you be interested in attending this course, please provide your details and your reasons for attending the course before noon on Wednesday, 23 January 2019.

Please use this link to apply:


Kindly be as descriptive as possible in answering questions as selections will be based on the answers you provided. If you know someone else who stands to benefit from this course, kindly forward this email to the relevant party. Please note that incomplete applications will not be considered. We look forward to receiving your applications.

On behalf of the organising committee

Molati Nonyane

Liaison Scientist 

African Center for Gene Technologies

Experimental Farm,  South Street

University of Pretoria


Tel: +27 12 420 6139/ +27 71 887 0438