Real-time PCR Facility at the CSIR

The Roche LightCycler instrument at the ACGT enables the quantitative real-time PCR amplification experiments. This helps to determine the amount of a specific DNA sequence of interest. There are major applications in the detection of GM food, but real-time PCR is also an important step for the validation of expression data generated by cDNA microarray screening and other gene discovery techniques.

The broad range of applications that run on LightCycler instruments has significantly contributed to the existing expertise in quantitative PCR. The instrument combines a thermal cycler platform with a fluorescence detection system, software modules and high-quality reagents, making it an ideal tool for research in the field of gene analysis.

Contact information

Tel: +27 12 841 3221

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